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Materials and methods Construction of the Chk YB b Neo
2021-07-13
Materials and methods Construction of the Chk-YB-1b-Neo vector. Approximately 107 plaques from a chicken genomic library established in EMBL3 vector (Clontech Palo Alto, CA) was screened and one clone containing a 12kb insert was selected. Digestion of this clone with SalI and EcoRI restriction enz
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Interestingly selective ETA blockade increased cutaneous
2021-07-13
Interestingly, selective ETA blockade increased cutaneous blood flow as measured by laser Doppler fluxmetry. This is a measure of total blood flow in the different vessels in the skin including venules, WAY-100635 maleate salt sale as well as arterioles. This finding is in accordance with the previ
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br Material and methods br Results br
2021-07-13
Material and methods Results Discussion Both progesterone and estradiol are major ovarian steroids crucial for the development and homeostasis of the female reproductive tract (Patel et al., 2015). In the cervix, estrogen softens the cervical stroma affecting the extracellular matrix (ECM)
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br Conflicts of interest br
2021-07-13
Conflicts of interest Acknowledgements This work was supported, in part or in whole, by the grants from the National Natural Science Foundation of China (11802056, 11772088), the China Postdoctoral Science Foundation (2018M640904), and the Fundamental Research Funds for the Central Universitie
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br Epac in cardiovascular pathophysiology
2021-07-13
Epac in cardiovascular pathophysiology Epac orchestrates signaling actors that regulate fundamental cellular functions and general biological effects. However, some important gaps still remain in the knowledge of the physiopathological role of Epac in the heart. So far, Epac has been implicated i
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Conventional assays rely on a comparable transformation of t
2021-07-13
Conventional assays rely on a comparable transformation of the target analytes and the structurally similar (but not identical) proxy substrates and are commonly used to measure individual enzyme activities in (pre-treated) intact sludge flocs (Burgess and Pletschke, 2008a, Gessesse et al., 2003, Yu
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A growing body of data
2021-07-13
A growing body of data indicates that endothelial NOS (eNOS) is a rate-limiting enzyme for the synthesis of nitric oxide (NO), its downstream 1670 molecule. High pathological concentrations of NO produced from inducible NO synthase (iNOS) induce apoptosis, whereas a reduction in the concentrations
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EW-7197 In general we observed that the
2021-07-13
In general, we observed that the enolase gene with highest mRNA levels was TVAG_043500, under all three iron concentration conditions. mRNA levels for this gene are very similar in normal and iron rich cultures (73.9 and 78.1 fold change, respectively), and higher in iron depleted cultures (118.3 fo
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br Material and methods br
2021-07-13
Material and methods Results Discussion Cysticerci located in host tissues are exposed to several effector mechanisms of the immune system (Flisser et al., 1986; Sciutto et al., 2000; Amit et al., 2011; Singh et al., 2013). In addition, the extensive uptake of host proteins by cysticerci ha
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br Materials and methods br Results
2021-07-12
Materials and methods Results Artemis has been reported to possess 5′–3′ exonuclease activity in vitro on ssDNA, as well as DNA-PK-dependent endonuclease activity on single-strand overhang and hairpin DNA structures [11]. However, enzymes within the metallo-β-lactamase family typically contain
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Studies of Jelski and Szmitkowski show that differences of a
2021-07-12
Studies of Jelski and Szmitkowski show that differences of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity between Naphthoquine phosphate of many cancers and healthy tissue may be one of the factors intensifying carcinogenesis [7]. Moreover, changes of ADH and ALDH activity i
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Previously to detect CDKL activity we used
2021-07-12
Previously, to detect CDKL5 activity, we used a radioactive phosphorylation assay with amphiphysin 1 protein as a substrate [,,]. However, it is necessary to establish a safe and rapid method for analyzing CDKL5 mutant activity. Here, we report a new method for investigating autophosphorylation acti
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It should be noted that there are other methods such
2021-07-12
It should be noted that there are other methods, such as artificial intelligence, like Artificial Neural Networks/ANN (such as PSO-ANN, ICA-ANN), Least Square Support Vector Machine/LSSVM, etc. [10], [11], [12], [13], [14], [15], [16], [17], [18], or molecular simulations, for modeling the fluid's v
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minocycline hydrochloride mg There are two general approache
2021-07-12
There are two general approaches to building Bayesian networks for the purpose of risk assessment. Researchers can implement static models that predict risk or survival at a snap-shot of time. For example, Loghmanpour et al. [10] created Bayesian network-based risk assessment models for patient data
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AdipoRon The effect of the simple
2021-07-12
The effect of the simple dopaminergic tonic/phasic model on cognitive processing has been investigated by a few studies (Nolan et al., 2004; Rosa et al., 2010). Rosa et al. (2010), using a Stroop task that requires different levels of cognitive flexibility and stability, observed an association betw
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